2017年中国部分地区白斑综合征病毒高变异区的序列分析

白斑综合征病毒(WSSV)自暴发以来给全球范围内的对虾养殖产业带来了巨大的损失。为了解我国凡纳滨对虾(Litopenaeus vannamei)养殖区WSSV的流行变异情况,选取2017年中国部分地区的42个WSSV阳性样本,对ORF14/15、ORF23/24、ORF75、ORF94和ORF125共5个可变区进行PCR特异性扩增,分析其序列的缺失变异和重复单元(Ru)中单核苷酸多态性的变化。研究结果显示,在ORF14/15的扩增中共出现4种缺失片段; ORF23/24只出现11 945 bp的缺失片段; ORF75扩增中,总RUs数目为3、4、9,其中45 bp的RUs在12、27、80位点发生多核苷酸多态性; ORF94的RUs数目为6,其各重复单元在48位发生单核苷酸多态性; ORF125的RUs数目为4、6、7不等,其各重复单元分别在20、27、50、53、61位发生碱基突变。研究结果表明,2017年样本中,WSSV在中国大部分地区均出现一定程度的缺失变异,其中部分可变区表现出缺失情况的稳定性,某些可变区的重复单元数目及SNP表现出地区的差异性以及不稳定性。     

番茄ShARPC5抗白粉病功能分析

【目的】白粉病（powdery mildew）是番茄生产上的重要病害,严重影响番茄的产量。番茄基因组测序工作的完成为抗病基因挖掘提供了重要的信息资源。ARP2/3（actin-related protein 2 and 3）复合体是肌动蛋白微丝骨架动力学的主要调控因子,能够参与包括响应外界胁迫等多种细胞学过程。本研究通过对番茄ARPC5（actin-related protein C5）进行克隆和抗病功能验证,为番茄基因组信息完善、抗病机制解析和分子育种等方面打下基础。【方法】从番茄LA1777（Solanum habrochaites）cDNA中PCR扩增ShARPC5,使用DNAMAN 6.0进行多序列比对;MEGA 6.0构建系统发育树;应用在线工具ProtComp v. 9.0进行亚细胞定位预测。利用实时荧光定量PCR（qRT-PCR）比较接种白粉菌（Oidium neolycopersici,On-Lz）后高感品种Moneymaker（MM）和高抗品种LA1777中番茄ARPC5的表达特征,分析白粉菌侵染与ARPC5表达的相关性。应用病毒诱导的基因沉默（virus-induced gene silencing,VIGS）技术进一步验证该基因在番茄中的抗病功能,观察沉默株和野生型株系接种后表型变化,利用台盼蓝和DAB染色法检测植株产生过敏性坏死和H₂O₂的能力,并检测ShARPC5沉默后一些与植物抗病相关标记基因的表达变化。采用农杆菌浸花法遗传转化拟南芥过表达ShARPC5植株,观察转基因和野生型株系接种后表型变化,并统计单病斑分生孢子数。【结果】从番茄品种LA1777中克隆到ShARPC5,编码132个氨基酸残基,包含一个保守的P16-Arc结构域。与番茄MM-白粉菌亲和互作相比,非亲和互作的番茄品种LA1777在接种白粉菌后,ShARPC5显著上调表达,尤其在接种后18 h。在番茄上沉默ShARPC5能够增加植株对白粉菌On-Lz的敏感性,防卫反应基因PR1b1显著下调表达。组织学观察显示与对照植株相比,ShARPC5沉默植株接种后诱导产生过敏性坏死和活性氧减少。在烟草上瞬时过表达ShARPC5能够诱导产生坏死斑。相反,在拟南芥上过表达ShARPC5能够增加植物的抗病性。【结论】ShARPC5是番茄响应白粉菌侵染的重要基因,可减轻番茄白粉病的发病程度,在番茄抗白粉病机理研究方面具有较大的应用价值,可作为番茄抗白粉病分子育种的一个候选基因。     

Genetic bases of source-,sink-,and yield-related traits revealed by genome-wide association study in Xian rice

The source-sink relationship determines the ultimate grain yield.We investigated the genetic basis of the relationship between source and sink and yield potential in rice.In two environments,we identified quantitative trait loci(QTL)associated with sink capacity(total spikelet number per panicle and thousand-grain weight),source leaf(flag leaf length,flag leaf width and flag leaf area),source-sink relationship(total spikelet number to flag leaf area ratio)and yield-related traits(filled grain number per panicle,panicle number per plant,grain yield per plant,biomass per plant,and harvest index)by genome-wide association analysis using 272 Xian(indica)accessions.The panel showed substantial variation for all traits in the two environments and revealed complex phenotypic correlations.A total of 70 QTL influencing the 11 traits were identified using 469,377 high-quality SNP markers.Five QTL were detected consistently in four chromosomal regions in both environments.Five QTL clusters simultaneously affected source,sink,source–sink relationship,and grain yield traits,probably explaining the genetic basis of significant correlations of grain yield with source and sink traits.We selected 24 candidate genes in the four consistent QTL regions by identifying linkage disequilibrium(LD)blocks associated with significant SNPs and performing haplotype analysis.The genes included one cloned gene(NOG1)and three newly identified QTL(qHI6,qTGW7,and qFLA8).These results provide a theoretical basis for high-yield rice breeding by increasing and balancing source–sink relationships using marker-assisted selection.     

秸秆还田对东北半干旱区玉米‖花生轮作系统土壤水热特性及作物产量的影响

以东北半干旱区玉米‖花生轮作系统为研究对象,试验设传统玉米单作、花生单作、不同秸秆还田量下(30%、60%和100%)玉米‖花生轮作5个处理。通过连续3年田间定位试验,研究玉米秸秆不同还田量对农田土壤水热特性、产量及土地当量比(LER)的影响。结果表明,与传统单作相比,秸秆还田处理玉米区播前0～40 cm土壤平均含水量提高了1.9%～3.9%,花生区提高了11.0%～13.9%;成熟期,秸秆还田花生区0:00～8:00平均地温比传统单作提高了0.2℃～1.1℃,10:00～22:00平均地温较传统单作降低了0.5℃～1.0℃。2016年和2017年玉米‖花生轮作系统LER为1.05～1.16,2018年各处理LER均小于1。吉林半干旱区玉米‖花生轮作系统进行秸秆还田改善了作物播前的土壤水分状况,但对于作物产量的影响在不同年份有所差异。     

小麦幼苗根系生理特性和解剖结构对根际pH的响应

为探讨根际pH 对冬小麦根系生长的影响，以半冬性小麦品种''矮抗58''（AK58）和''百农4199''（BN4199）为材料，采用水培试验，设置3个pH 水平（4.0、6.5、9.0，以6.5为对照），研究根际pH 对冬小麦根系抗氧化酶系统的影响及根系解剖结构对pH 的响应。结果表明：酸碱胁迫下，植株地上部及根系生物量、抗氧化酶活性较对照下降，且酸胁迫较碱胁迫下降幅度大。两品种根冠比表现为pH 4.0＜pH 6.5＜pH 9.0，丙二醛（MDA）含量表现为pH 6.5＜pH 9.0＜pH 4.0。与对照相比，两品种碱性条件下根系结构受损较小，而酸性条件下受损较大。相关分析表明地上部和根系干物质积累量与抗氧化酶活性呈显著正相关，与根系MDA含量呈极显著负相关。可见，酸碱胁迫下，保持较高的抗氧化酶活性有利于保持根系生长，从而提高小麦适应不良根际环境的能力。     

超级稻甬优12产量1000kg/667m~2手插栽培技术初步集成

以甬优12在居于5 300℃积温线的浙江中部新昌县的试验实践为基础,提出了1 000 kg/667 m2手插栽培的基本技术思路、群体生育指标及主要集成技术。     

C语言在线评价与测试系统的开发与探讨

随着网络教育的发展,接受网络教育和考试认证的人越来越多,在线考试正在得以迅速发展.本文简要介绍在线系统工作原理和系统配置.     

Movement and survival of corn rootworm in seed mixtures of SmartStax® insect-protected corn

SmartStax^® insect-protected corn (Zea mays L.) contains genes for six Bacillus thuringiensis (Bt) proteins controlling both lepidopteran pests and the corn rootworm complex (Diabrotica spp.). The properties of SmartStax, particularly the multiple effective modes of action (i.e., each Bt protein provides a high level of control of the target pests with a low probability of cross-resistance among the proteins), have provided the opportunity to add to previously approved structured refuge options by combining the non-Bt refuge seed with SmartStax seed in a seed mix. Seed mixes ensure that a refuge is present in every Bt field, remove concerns about grower compliance with refuge requirements, and provide grower convenience. However, seed mixes could increase the likelihood that larval insects move between Bt and non-Bt plants and vice versa. Assessing the insect resistance management (IRM) value of a seed mix refuge requires an assessment of the amount of larval movement, and the consequences of that movement, for the key target pests. The studies here present such data for control of corn rootworm by SmartStax corn, which contains the rootworm-active protein Cry3Bb1 and the binary protein Cry34Ab1/Cry35Ab1. In a growth chamber experiment, SmartStax was most effective against first instars and significantly effective against second instars, but did not control third instars. In a field study of movement from a heavily infested non-Bt plant onto surrounding plants, a larger percentage of insects successfully dispersed from the infested plant when the surrounding plants were non-Bt plants than when they were SmartStax plants. A paired-plant study showed that few larvae migrated from infested SmartStax plants and survived on nearby non-Bt plants; larvae that migrated from infested non-Bt plants had low survival if the adjacent plants were SmartStax. Replicated field studies of plant-to-plant movement indicated that the non-Bt plants in a 5% or 10% seed mix consistently supported large populations of susceptible insects and represented a productive refuge, whereas the SmartStax plants had few or no survivors. The timing of emergence from seed mix plots containing 5% or 10% non-Bt plants was more similar to that of the non-Bt plots than that of the SmartStax plots. Thus, the available growth chamber and field data indicate that a seed mix of 5% or more will provide an effective refuge for corn rootworm in SmartStax corn.     

Antioxidant,anti-amylase and anti-glycation potential of brans of some Sri Lankan traditional and improved rice (Oryza sativa L.) varieties

Brans of 23 traditional and 12 improved (both red and white) rice varieties in Sri Lanka were screened for anti-amylase and anti-glycation activities in vitro. Varieties which showed the highest inhibitory activities at screening were further investigated for anti-glucosidase and glycation reversing as anti-diabetic properties. The same varieties were studied for selected antioxidant properties. Significantly high anti-amylase and anti-glycation activities were observed for bran extracts of red varieties compared to white varieties at screening. Traditional red rice varieties, Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti, exhibited significant and dose dependent anti-amylase, anti-glycation and glycation reversing activities. These varieties also showed marked antioxidant properties. It is concluded that brans of Sri Lankan traditional red rice varieties Masuran, Sudu Heeneti, Dik Wee and Goda Heeneti may be potential food supplements for diabetes.